Human Neuronal Nitric Oxide Synthase (nNOS) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E13872h

Sensitivity

0.039 IU/mL

Detection Range

0.156 IU/mL-10 IU/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

nitric oxide synthase 1 (neuronal), IHPS1, NOS, nNOS, nitric oxide synthase 1, neuronal

Recovery

The recovery of mouse TCC C5b-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	97	92-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TCC C5b-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	88
	Range %	84-96
1:200	Average %	98
	Range %	91-105
1:400	Average %	95
	Range %	91-99
1:800	Average %	92
	Range %	88-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sample Type

Serum, Cell culture supeatants, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P29475
Tissue Specificity	Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected

Applications	ELISA
Research Area	NOS1

Storage	Store at 2-8°C. Please refer to protocol.

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