Human Neurotrophin-3,NT-3 ELISA KIT

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E04686h

Sensitivity

0.31 ng/mL

Detection Range

1.25 ng/mL-80 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

Neurotrophin-3,NT-3

Recovery

The recovery of bovine C5a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-103
EDTA plasma (n=4)	90	86-94

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine C5a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	88-93
1:2	Average %	101
	Range %	97-104
1:4	Average %	94
	Range %	88-99
1:8	Average %	93
	Range %	89-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sample Type

Serum, Cell culture supernatants, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P20783
Tissue Specificity	Brain and peripheral tissues.

Applications	ELISA
Research Area	NT-3

Storage	Store at 2-8°C. Please refer to protocol.

Reference	MicroRNA-182 aggravates cerebral ischemia injury by targeting inhibitory member of the ASPP family (iASPP) Yi H.et al,Arch Biochem Biophys.,S0003-9861(16)30141-2

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