Human NPL(N-acetylneuraminate lyase) ELISA Kit

Mfr.No.

ELK0324

Sensitivity

0.097 ng/mL

Detection Range

0.32-20 ng/mL

Assay Duration

3.5h

Assay Type

Sandwich

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NPL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	92-105%	83-92%	89-98%	85-94%
EDTA Plasma(n=5)	95-104%	79-92%	82-95%	86-97%
Heparin Plasma(n=5)	85-92%	89-103%	97-105%	91-99%

Standard Curve

Concentration (ng/mL)	OD	Corrected OD
20.00	2.121	2.033
10.00	1.783	1.695
5.00	1.136	1.048
2.50	0.875	0.787
1.25	0.457	0.369
0.63	0.365	0.277
0.32	0.205	0.117
0.00	0.088	0.000

Sample Type

Serum, Plasma, Other biological fluids

Limit Of Detection

20 ng/mL

Precision

Matrices listed below were spiked with certain level of recombinant NPL and the recovery rates were calculated by comparing the measured value to the expected amount of NPL in samples.

Matrix	Recovery range	Average
serum(n=5)	82-95%	88%
EDTA Plasma(n=5)	80-95%	87%
Heparin Plasma(n=5)	86-99%	92%

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NPL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NPL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NPL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NPL in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Manual
MSDS

UniProt ID	Q9BXD5

Research Area	Enzyme & Kinase;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15209169	ELK0324	48T	$320		Add to Cart
BHE15209169	ELK0324	96T	$458		Add to Cart

Human NPL(N-acetylneuraminate lyase) ELISA Kit

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