Human NTXI(Cross Linked N-Telopeptide Of Type I Collagen) ELISA Kit

Mfr.No.

ELK1843

Sensitivity

0.94 ng/mL

Detection Range

3.13-200 ng/mL

Reactivity

Human

Assay Duration

Assay Type

Competitive Inhibition

Alternative Names

NTX-I; NTX1

Recovery

Matrices listed below were spiked with certain level of recombinant NTXI and the recovery rates were calculated by comparing the measured value to the expected amount of NTXI in samples.

Matrix	Recovery range	Average
serum(n=5)	82-95%	88%
EDTA plasma(n=5)	80-95%	87%
Heparin plasma(n=5)	86-99%	92%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NTXI and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	79-94%	87-96%	82-95%	89-102%
EDTA plasma(n=5)	85-92%	87-103%	90-98%	95-102%
Heparin plasma(n=5)	83-97%	81-103%	89-101%	81-98%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
200.00	0.197
100.00	0.310
50.00	0.527
25.00	0.842
12.50	1.168
6.25	1.457
3.13	1.796
0.00	2.112

Sample Type

Cell lysates, Serum, Cell culture supernatants, Tissue homogenates, Plasma, Other biological fluids

Limit Of Detection

200 ng/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human NTXI protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NTXI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NTXI in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

Applications	ELISA
Research Area	Metabolic pathway;Bone metabolism;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15200739	ELK1843	48T	$320		Add to Cart
BHE15200739	ELK1843	96T	$458		Add to Cart

Human NTXI(Cross Linked N-Telopeptide Of Type I Collagen) ELISA Kit

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