Human Osteoclast-associated immunoglobulin-like receptor(OSCAR) ELISA kit

Human Osteoclast-associated immunoglobulin-like receptor(OSCAR) ELISA kit

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Mfr.No.

CSB-EL017255HU

Alternative Names

osteoclast associated, immunoglobulin-like receptor, MGC33613, PIGR3, osteoclast associated receptor OSCAR-S1, osteoclast associated receptor OSCAR-S2, osteoclast-associated receptor, polymeric immunoglobulin receptor 3

Reactivity

Human

Detection Range

6.25 ng/mL-400 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human TFF2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	98	90-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TFF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	87
	Range %	80-92
1:2	Average %	95
	Range %	90-100
1:4	Average %	97
	Range %	90-103
1:8	Average %	92
	Range %	88-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
2000	2.026	2.007	2.017	1.920
1000	1.364	1.306	1.335	1.238
500	0.788	0.724	0.756	0.659
250	0.457	0.427	0.442	0.345
125	0.262	0.246	0.254	0.157
62.5	0.175	0.167	0.171	0.074
31.25	0.119	0.114	0.117	0.020
0	0.101	0.093	0.097	?

Species

Homo sapiens

Sensitivity

1.56 ng/mL

Limit Of Detection

450 nm

UniProt ID	Q8IYS5
Antigen	osteoclast associated, immunoglobulin-like receptor

Applications

ELISA

Research Area

OSCAR

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503647	CSB-EL017255HU	96T	$595	Add to Cart
BHE10503647	CSB-EL017255HU	5x96T	$2856	Add to Cart
BHE10503647	CSB-EL017255HU	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.