Human ovalbumin specific IgG,OVA sIgG ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E10127h

Sensitivity

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Detection Range

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Reactivity

Human

Assay Duration

1-5h

Sample Volume

50-100ul

Alternative Names

ovalbumin specific IgG,OVA sIgG

Recovery

The recovery of rat IL-21 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-101
EDTA plasma (n=4)	92	88-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-21 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	85-96
1:2	Average %	89
	Range %	84-95
1:4	Average %	103
	Range %	99-108
1:8	Average %	99
	Range %	94-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Applications	ELISA
Research Area	OVA sIgG

Storage	Store at 2-8°C. Please refer to protocol.

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