Human Pancreatic Polypeptide

Mfr.No.

E0032Hu

Sensitivity

10.48ng/L

Detection Range

20-4500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Human Pancreatic Polypeptide (also known as PP) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Obinepitide;Pancreatic hormone;Pancreatic icosapeptide;Pancreatic polypeptide;Pancreatic prohormone;PH;PI;PP;PPY;

Molecular Weight

10445

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E0032Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1033.9	53.43	5.2
Intra-Assay	2	18	152.3	7.62	5
Intra-Assay	3	18	399.8	24.3	6.1

Background

Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions. The physiological role for the icosapeptide has not yet been elucidated.

Protocol

These standard curves of E0032Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.179	2.186	2.148
	2.193
1200ng/L	1.847	1.78	1.742
	1.712
600ng/L	1.261	1.265	1.227
	1.269
300ng/L	0.701	0.673	0.635
	0.646
150ng/L	0.412	0.388	0.35
	0.365
0ng/L	0.03	0.038	0
	0.046

Gene ID	5539
UniProt ID	P01298

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101768	E0032Hu	96T	$458		Add to Cart
BHE12101768	E0032Hu	48T	$320		Add to Cart

Human Pancreatic Polypeptide - PP ELISA Kit

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