Human Protamine 2 - PRM2 ELISA Kit

Mfr.No.

E6449Hu

Sensitivity

0.054ng/mL

Detection Range

0.1-40ng/mL

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Basic nuclear protein HPI1;Basic nuclear protein HPI2;Basic nuclear protein HPS1;Basic nuclear protein HPS2;P2';P2'';PRM 2;PRM2;Protamine-2;Sperm histone HP2;Sperm histone HP3;Sperm histone HP4;Sperm histone P2;Sperm protamine P2;Sperm protamine P3;Sperm protamine P4

Molecular Weight

13051

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E6449Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	10.7	0.44	4.1
Intra-Assay	2	18	19.7	0.75	3.8
Intra-Assay	3	18	8.3	0.34	4.1

Background

Protamines substitute for histones in the chromatin of sperm during the haploid phase of spermatogenesis. They compact sperm DNA into a highly condensed, stable and inactive complex.

Protocol

These standard curves of E6449Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/mL	1.727	1.792	1.75
	1.858
12ng/mL	0.966	1.008	0.966
	1.05
6ng/mL	0.511	0.502	0.46
	0.494
3ng/mL	0.207	0.244	0.202
	0.281
1.5ng/mL	0.125	0.09	0.048
	0.054
0ng/mL	0.033	0.042	0
	0.051

Gene ID	5620
UniProt ID	P04554

Research Area	Epigenetics and Nuclear Signaling

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107811	E6449Hu	48T	$320		Add to Cart
BHE12107811	E6449Hu	96T	$458		Add to Cart

Human Protamine 2 - PRM2 ELISA Kit

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