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Home / ELISA Kit

Human Pulmonary surfactant-associated protein C,SP-C ELISA Kit

SKU : BHE10504073

This Rat IgM ELISA Kit was designed for the quantitative measurement of Rat IgM protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 6.25 ng/mL-1600 ng/mL and the sensitivity is 6.25 ng/mL .

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Mfr.No.

CSB-E10135h

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Recovery

The recovery of rat IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type		Average % Recovery		Range
Serum (n=5)		94		92-97
EDTA plasma (n=4)		93		89-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?		Sample		Serum(n=4)
1:1000		Average %		99
		Range %		95-104
1:2000		Average %		90
		Range %		86-95
1:4000		Average %		89
		Range %		83-93
1:8000		Average %		100
		Range %		95-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
1600	0.234	0.227	0.231
400	0.433	0.412	0.423
100	0.678	0.669	0.674
25	1.256	1.271	1.264
6.25	1.698	1.784	1.741
0	2.031	2.049	2.040

Species

Homo sapiens

Sample Type

Serum, Urine, Cell Culture Supernates, Plasma

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

UniProt ID	P11686

Applications	ELISA
Research Area	SFTPC

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price	Quantity
BHE10504073	CSB-E10135h	96T	$695		Add to Cart
BHE10504073	CSB-E10135h	5x96T	$3336		Add to Cart
BHE10504073	CSB-E10135h	10x96T	$6602.50		Add to Cart

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