Human ReverseTri-iodothyronine,rT3 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E09485h

Sensitivity

75 pg/mL

Detection Range

75 pg/mL-1200 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Competitive

Sample Volume

50-100ul

Alternative Names

ReverseTri-iodothyronine,rT3

Recovery

The recovery of mouse CK-MM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	85-99
EDTA plasma (n=4)	100	95-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CK-MM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	87
	Range %	83-98
1:2	Average %	98
	Range %	92-103
1:4	Average %	104
	Range %	98-108
1:8	Average %	93
	Range %	87-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sample Type

Serum, Cell culture supernatants, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	rT3

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Study the Changes of Thyroid Hormones Levels Following Major Urological Surgery Fayhaa M. Khaleel. et al,Baghdad Science Journal ,Accepted November 25, 2014 The Effect of Anesthesia on Thyroid Hormones and Cortisol Levels Following Major Urological Surgery Fayhaa M. Khaleel .et al,Baghdad University,/

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