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Home / ELISA Kit

Human Sestrin-1 (SESN1) ELISA Kit

SKU : BHE13702903

Only the smaller transcript was transiently induced by p53 or by genotoxic agents in a p53-dependent manner in a colon carcinoma cell line. The deduced proteins, which are 99% identical to the mouse proteins, contain 551, 491, and 426 amino acids, respectively. RT-PCR analysis detected induction of T2 in response to p53 or genotoxic stress; however, T1 was not induced and T3 was only weakly induced. Western blot analysis showed induction of a predominant 55-kD T2 nuclear protein rather than the 68-kD T1 protein or the 48-kD T3 protein. Sequence analysis predicted, and EMSA and reporter analysis confirmed, that PA26 has a p53-binding site within intron 2. Serum starvation experiments suggested that PA26 may be a member of the growth arrest- and DNA damage-inducible, or GADD, gene family.

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Mfr.No.	KTE60687
Description	This Human Sestrin-1 (SESN1) ELISA Kit employs a two-site sandwich ELISA to quantitate SESN1.
Alternative Names	SESN1; MGC138241; MGC142129; PA26; RP11-787I22.1; SEST1; p53 regulated PA26 nuclear protein
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Sestrin-1 microplate Human Sestrin-1 standard Human Sestrin-1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Sestrin-1 (SESN1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SESN1. No significant cross-reactivity or interference between Human SESN1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Only the smaller transcript was transiently induced by p53 or by genotoxic agents in a p53-dependent manner in a colon carcinoma cell line. The deduced proteins, which are 99% identical to the mouse proteins, contain 551, 491, and 426 amino acids, respectively. RT-PCR analysis detected induction of T2 in response to p53 or genotoxic stress; however, T1 was not induced and T3 was only weakly induced. Western blot analysis showed induction of a predominant 55-kD T2 nuclear protein rather than the 68-kD T1 protein or the 48-kD T3 protein. Sequence analysis predicted, and EMSA and reporter analysis confirmed, that PA26 has a p53-binding site within intron 2. Serum starvation experiments suggested that PA26 may be a member of the growth arrest- and DNA damage-inducible, or GADD, gene family.
Notes	This Human Sestrin-1 (SESN1) ELISA Kit employs a two-site sandwich ELISA to quantitate SESN1 in samples. An antibody specific for SESN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySESN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SESN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SESN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	140742
Antigen	SESN1
Tag	SESN1
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Sestrin-1 (SESN1) ELISA Kit employs a two-site sandwich ELISA to quantitate SESN1 in samples. An antibody specific for SESN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySESN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SESN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SESN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702903	KTE60687	48 T	$339		Add to Cart
BHE13702903	KTE60687	96 T	$589		Add to Cart
BHE13702903	KTE60687	96 Tx5	$2629		Add to Cart
BHE13702903	KTE60687	96 Tx50	$24569		Add to Cart

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