Human Sphingosine 1 Phosphate Receptor 3

Mfr.No.

E6749Hu

Sensitivity

0.018ng/ml

Detection Range

0.05-20ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Endothelial differentiation G-protein coupled receptor 3;S 1PR3;S1P receptor 3;S1P receptor Edg-3;S1P3;S1PR3;Sphingosine 1-phosphate receptor 3;Sphingosine 1-phosphate receptor Edg-3

Molecular Weight

42250

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E6749Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	7.7	0.39	5
Intra-Assay	2	18	4.1	0.15	3.6
Intra-Assay	3	18	2.5	0.1	3.8

Background

Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis.

Protocol

These standard curves of E6749Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	1.944	1.97	1.935
	1.996
6ng/ml	1.31	1.416	1.381
	1.522
3ng/ml	0.608	0.688	0.653
	0.769
1.5ng/ml	0.413	0.366	0.331
	0.319
0.75ng/ml	0.116	0.144	0.109
	0.171
0ng/ml	0.027	0.035	0
	0.042

Gene ID	1903
UniProt ID	Q99500

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115598	E6749Hu	48T	$320		Add to Cart
BHE12115598	E6749Hu	96T	$458		Add to Cart

Human Sphingosine 1 Phosphate Receptor 3 - S1PR3 ELISA Kit

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