Human Thromboxane B2,TX-B2 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08046h

Sensitivity

2.34 pg/mL

Detection Range

9.38 pg/mL-600 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

Thromboxane B2,TX-B2

Recovery

The recovery of pig GNRH2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	97	89-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig GNRH2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	81-92
1:2	Average %	96
	Range %	90-105
1:4	Average %	97
	Range %	92-105
1:8	Average %	94
	Range %	88-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sample Type

Serum, Cell culture supernatants, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	TX-B2

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Anti-Müllerian hormone as a diagnostic tool for ovarian remnant syndrome in bitches. Turna Yilmaz Ouml. et al,Vet Res Commun,2015 Sep;39(3):159-162. Anti-inflammatory Activity of Magnesium Isoglycyrrhizinate Through Inhibition of Phospholipase A2/Arachidonic Acid Pathway Xie C et al,Inflammation,2015 Feb 19. The effect of aspirin on circulating netrin-1 levels in humans is dependent on the inflammatory status of the vascular endothelium Layne K.et al,Oncotarget,Nov 17 Glucocorticoids impair platelet thromboxane biosynthesis in community-acquired pneumonia Cangemi R.et al,Pharmacol Res.,Mar 22

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