Human Transforming Growth Factor alphalpha

Mfr.No.

E0136Hu

Sensitivity

5.11ng/L

Detection Range

10-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Transforming Growth Factor Alpha (also known as TGF-A) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

EGF-like TGF;ETGF;Protransforming growth factor alpha Transforming growth factor alpha;TGF A;TGF alpha;TGF type 1;TGFA;TGF-alpha

Molecular Weight

17006

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E0136Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	196.9	8.85	4.5
Intra-Assay	2	18	715.5	18.87	2.6
Intra-Assay	3	18	526.9	24.42	4.6

Background

TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.

Protocol

These standard curves of E0136Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.106	2.163	2.125
	2.219
800ng/L	1.458	1.393	1.355
	1.328
400ng/L	0.659	0.668	0.631
	0.678
200ng/L	0.416	0.361	0.323
	0.306
100ng/L	0.202	0.196	0.158
	0.19
0ng/L	0.037	0.038	0
	0.039

Gene ID	7039
UniProt ID	P01135

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101871	E0136Hu	96T	$458		Add to Cart
BHE12101871	E0136Hu	48T	$320		Add to Cart

Human Transforming Growth Factor alphalpha - TGF-A ELISA Kit

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