Human TRPV4(Transient Receptor Potential Cation Channel Subfamily V, Member 4) ELISA Kit

Mfr.No.

ELK0233

Sensitivity

5.3 pg/mL

Detection Range

15.63-1000 pg/mL

Assay Duration

3.5h

Assay Type

Sandwich

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TRPV4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	89-97%	85-94%	79-96%	85-99%
EDTA Plasma(n=5)	87-99%	85-96%	87-101%	89-102%
Heparin Plasma(n=5)	93-102%	90-99%	95-106%	87-101%

Standard Curve

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.266	2.162
500.00	1.699	1.595
250.00	1.132	1.028
125.00	0.895	0.791
62.50	0.525	0.421
31.25	0.354	0.250
15.63	0.230	0.126
0.00	0.104	0.000

Sample Type

Serum, Plasma, Tissue homogenates, Cell lysates, Breast milk, Cell culture supernates, Other biological fluids

Limit Of Detection

1000 pg/mL

Precision

Matrices listed below were spiked with certain level of recombinant TRPV4 and the recovery rates were calculated by comparing the measured value to the expected amount of TRPV4 in samples.

Matrix	Recovery range	Average
serum(n=5)	87-99%	93%
EDTA Plasma(n=5)	82-98%	90%
Heparin Plasma(n=5)	90-107%	98%

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TRPV4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TRPV4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TRPV4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TRPV4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Manual
MSDS

UniProt ID	Q9HBA0

Research Area	Signal transduction;

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE15209098	ELK0233	48T	$320		Add to Cart
BHE15209098	ELK0233	96T	$458		Add to Cart

Human TRPV4(Transient Receptor Potential Cation Channel Subfamily V, Member 4) ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human TRPV4(Transient Receptor Potential Cation Channel Subfamily V, Member 4) ELISA Kit

Rate it now..!!