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Home / ELISA Kit

Human Very low density lipoprotein (VLDL) ELISA Kit

SKU : BHE13702270

VLDL is a type of lipoprotein made by the liver. VLDL is one of the five major groups of lipoproteins (chylomicrons, VLDL, intermediate-density lipoprotein, low-density lipoprotein, high-density lipoprotein) that enable fats and cholesterol to move within the water-based solution of the bloodstream. VLDL is assembled in the liver from cholesterol and apolipoproteins.
VLDL is converted in the bloodstream to low-density lipoprotein (LDL). VLDL particles have a diameter of 30-80 nm. VLDL transports endogenous products, whereas chylomicrons transport exogenous (dietary) products.VLDL transports endogenous triglycerides, phospholipids, cholesterol, and cholesteryl esters. It functions as the body's internal transport mechanism for lipids.

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Mfr.No.	KTE60050
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Very low density lipoprotein (VLDL) ELISA Kit employs a two-site sandwich ELISA to quantitate VLDL.
Alternative Names	VLDL; Pre-beta-Lipoprotein; Pre-Beta Lipoprotein
Detection Method	Colorimetric
Kit Components	Human Very low density lipoprotein microplate Human Very low density lipoprotein standard Human Very low density lipoprotein detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Very low density lipoprotein (VLDL) ELISA Kit has high sensitivity and excellent specificity for detection of Human VLDL. No significant cross-reactivity or interference between Human VLDL and analogues was observed.
Calibration Range	Please inquire
Sample Type	Serum, Cell culture supernatants, Plasma, Other biological fluids
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	VLDL is a type of lipoprotein made by the liver. VLDL is one of the five major groups of lipoproteins (chylomicrons, VLDL, intermediate-density lipoprotein, low-density lipoprotein, high-density lipoprotein) that enable fats and cholesterol to move within the water-based solution of the bloodstream. VLDL is assembled in the liver from cholesterol and apolipoproteins. VLDL is converted in the bloodstream to low-density lipoprotein (LDL). VLDL particles have a diameter of 30-80 nm. VLDL transports endogenous products, whereas chylomicrons transport exogenous (dietary) products.VLDL transports endogenous triglycerides, phospholipids, cholesterol, and cholesteryl esters. It functions as the body's internal transport mechanism for lipids.
Notes	This Human Very low density lipoprotein (VLDL) ELISA Kit employs a two-site sandwich ELISA to quantitate VLDL in samples. An antibody specific for VLDL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVLDL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VLDL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VLDL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Very low density lipoprotein (VLDL) ELISA Kit employs a two-site sandwich ELISA to quantitate VLDL in samples. An antibody specific for VLDL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVLDL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VLDL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VLDL bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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