Mouse 5-Nucleotidase,5-NT ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E04682m

Sensitivity

0.031 mU/mL

Detection Range

0.125 mU/mL-8 mU/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

5-Nucleotidase,5-NT

Recovery

The recovery of human P53 antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	83-96
EDTA plasma (n=4)	94	87-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human P53 antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-105
1:2	Average %	94
	Range %	84-99
1:4	Average %	99
	Range %	90-101
1:8	Average %	92
	Range %	82-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q61503

Applications	ELISA
Research Area	5-NT

Storage	Store at 2-8°C. Please refer to protocol.

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