Mouse angiotensinogen (aGT) ELISA Kit

Mouse angiotensinogen (aGT) ELISA Kit

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Mfr.No.

CSB-E08566m

Sensitivity

0.78 ng/mL

Detection Range

3.12 ng/mL-200 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

angiotensinogen (serpin peptidase inhibitor, clade A, member 8), ANHU, FLJ92595, FLJ97926, SERPINA8, alpha-1 antiproteinase, antitrypsin, angiotensin I, angiotensin II, angiotensinogen, pre-angiotensinogen, serine (or cysteine) proteinase inhibitor

Recovery

The recovery of mouse BMP-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	91-96
EDTA plasma (n=4)	95	93-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse BMP-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-92
1:2	Average %	103
	Range %	98-106
1:4	Average %	92
	Range %	89-97
1:8	Average %	100
	Range %	97-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
40	2.449	2.440	2.445	2.295
20	1.788	1.818	1.803	1.653
10	1.196	1.181	1.189	1.039
5	0.584	0.604	0.594	0.444
2.5	0.424	0.415	0.420	0.270
1.25	0.321	0.312	0.317	0.167
0.625	0.272	0.255	0.264	0.114
0	0.152	0.148	0.150	?

Species

Mus musculus

Sample Type

Serum, Cell culture supernatants, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P11859
Tissue Specificity	Expressed by the liver and secreted in plasma.

Applications	ELISA
Research Area	AGT

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505144	CSB-E08566m	96T	$835	Add to Cart
BHE10505144	CSB-E08566m	5x96T	$4008	Add to Cart
BHE10505144	CSB-E08566m	10x96T	$7932.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.