Mouse BPI(Bactericidal/Permeability Increasing Protein) ELISA Kit

Mfr.No.

ELK3629

Sensitivity

13.3 pg/mL

Detection Range

31.25-2000 pg/mL

Reactivity

Mouse

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

CAP 57

Recovery

Matrices listed below were spiked with certain level of recombinant BPI and the recovery rates were calculated by comparing the measured value to the expected amount of BPI in samples.

Matrix	Recovery range	Average
serum(n=5)	78-92%	85%
EDTA plasma(n=5)	90-105%	97%
Heparin plasma(n=5)	80-97%	88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BPI and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	81-94%	93-107%	93-102%	91-105%
EDTA plasma(n=5)	92-106%	93-105%	85-99%	90-99%
Heparin plasma(n=5)	95-106%	87-101%	82-97%	87-102%

Typical Data

<table> <tbody><tr> <th>Concentration (pg/mL)</th> <th>OD</th> <th>Corrected OD</th> </tr> <tr> <td>2000.00</td> <td>2.401</td> <td>2.306</td> </tr> <tr> <td>1000.00</td> <td>1.653</td> <td>1.558</td> </tr> <tr> <td>500.00</td> <td>1.115</td> <td>1.020</td> </tr> <tr> <td>250.00</td> <td>0.797</td> <td>0.702</td> </tr> <tr> <td>125.00</td> <td>0.553</td> <td>0.458</td> </tr> <tr> <td>62.50</td> <td>0.381</td> <td>0.286</td> </tr> <tr> <td>31.25</td> <td>0.228</td> <td>0.133</td> </tr> <tr> <td>0.00</td> <td>0.095</td> <td>0.000</td> </tr> </tbody></table>

Sample Type

Serum, Plasma, Other biological fluids

Limit Of Detection

2000 pg/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BPI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BPI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse BPI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BPI in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

<a href="https://s3.us-west-1.amazonaws.com/s3.biohippo.com/elisakits/elk/ELK3629-1.pdf" target="_blank">Datasheet</a>

UniProt ID	Q67E05

Applications	ELISA
Research Area	Infection immunity;Dermatology;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15206723	ELK3629	48T	$320		Add to Cart
BHE15206723	ELK3629	96T	$458		Add to Cart

Mouse BPI(Bactericidal/Permeability Increasing Protein) ELISA Kit

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