Mouse CD40L(Cluster Of Differentiation 40 Ligand) ELISA Kit

Mfr.No.

ELK2308

Sensitivity

6.7 pg/mL

Detection Range

15.63-1000 pg/mL

Reactivity

Mouse

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

CD154; TRAP; HIGM1; IGM; IMD3; TBAM; T-BAM; TNFSF5; Gp39; TNF Superfamily Member 5; Hyper-IgM Syndrome; TNF-Related Activation Protein; T-Cell B-Cell Activating Molecule

Recovery

Matrices listed below were spiked with certain level of recombinant CD40L and the recovery rates were calculated by comparing the measured value to the expected amount of CD40L in samples.

Matrix	Recovery range	Average
serum(n=5)	80-95%	87%
EDTA plasma(n=5)	82-97%	89%
Heparin plasma(n=5)	95-108%	101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CD40L and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	89-97%	93-102%	81-96%	93-106%
EDTA plasma(n=5)	86-93%	91-105%	87-98%	89-94%
Heparin plasma(n=5)	93-102%	93-106%	87-101%	91-104%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.172	2.085
500.00	1.515	1.428
250.00	1.135	1.048
125.00	0.847	0.760
62.50	0.543	0.456
31.25	0.355	0.268
15.63	0.258	0.171
0.00	0.087	0.000

Sample Type

Cell lysates, Serum, Tissue homogenates, Plasma, Other biological fluids

Limit Of Detection

1000 pg/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CD40L. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CD40L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CD40L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CD40L in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

UniProt ID	P27548

Applications	ELISA
Research Area	Signal transduction;CD & Adhesion molecule;Apoptosis;Tumor immunity;Infection immunity;Immune molecule;

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE15206978	ELK2308	48T	$320		Add to Cart
BHE15206978	ELK2308	96T	$458		Add to Cart

Mouse CD40L(Cluster Of Differentiation 40 Ligand) ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Mouse CD40L(Cluster Of Differentiation 40 Ligand) ELISA Kit

Rate it now..!!