Mouse Claudin-5(CLDN5) ELISA kit

Mouse Claudin-5(CLDN5) ELISA kit

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Mfr.No.

CSB-EL005507MO

Sensitivity

4.68 pg/mL

Detection Range

18.75 pg/mL-1200 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

claudin 5, AWAL, BEC1, CPETRL1, TMVCF, OTTHUMP00000197372, OTTHUMP00000197819, androgen withdrawal and apoptosis induced protein RVP1-like, transmembrane protein deleted in velocardiofacial syndrome

Recovery

The recovery of rat NT-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-102
EDTA plasma (n=4)	100	95-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat NT-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-94
1:2	Average %	93
	Range %	88-98
1:4	Average %	96
	Range %	90-102
1:8	Average %	99
	Range %	95-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
3000	2.268	2.250	2.259	2.150
1500	1.845	1.871	1.858	1.749
750	1.464	1.492	1.478	1.369
375	1.135	1.149	1.142	1.033
187.5	0.704	0.724	0.714	0.605
94	0.438	0.457	0.448	0.339
47	0.287	0.294	0.291	0.182
0	0.108	0.110	0.109	?

Species

Mus musculus

Sample Type

Cell lysates, Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	O54942
Tissue Specificity	Widely expressed with highest levels in the lung.

Applications	ELISA
Research Area	CLDN5

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505395	CSB-EL005507MO	96T	$695	Add to Cart
BHE10505395	CSB-EL005507MO	5x96T	$3336	Add to Cart
BHE10505395	CSB-EL005507MO	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.