Mouse Collagen alpha-1(I) chain (COL1A1) ELISA kit

Mouse Collagen alpha-1(I) chain (COL1A1) ELISA kit

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Mfr.No.

CSB-EL005727MO

Sensitivity

0.078 ng/mL

Detection Range

0.312 ng/mL-20 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

collagen, type I, alpha 1, OI4, alpha 1 type I collagen, collagen alpha 1 chain type I, collagen of skin, tendon and bone, alpha-1 chain, pro-alpha-1 collagen type 1,_x000D_ Collagen alpha-1(1) chain (COL1A1),_x000D_ Collagen alpha-1(I) chain (COL1A1), collagen, type I, alpha 1, OI4, alpha 1 type I collagen, collagen alpha 1 chain type I, collagen of skin, tendon and bone, alpha-1 chain, pro-alpha-1 collagen type 1,_x000D_ Collagen alpha-1(1) chain (COL1A1),_x000D_ Collagen alpha-1(I) chain (COL1A1)

Recovery

The recovery of human NTX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-106
EDTA plasma (n=4)	92	88-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human NTX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-93
1:2	Average %	98
	Range %	94-102
1:4	Average %	102
	Range %	98-106
1:8	Average %	95
	Range %	92-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

nM BCE	OD1	OD2	Average	Corrected
500	1.983	1.976	1.980	1.850
250	1.446	1.550	1.498	1.368
125	1.011	1.093	1.052	0.922
62.5	0.616	0.625	0.621	0.491
31.25	0.374	0.361	0.368	0.238
15.6	0.272	0.274	0.273	0.143
7.8	0.204	0.215	0.210	0.080
0	0.131	0.129	0.130	?

Species

Mus musculus

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P11087
Tissue Specificity	Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.

Applications	ELISA
Research Area	COL1A1

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Identification of vitamin D sensitive pathways during lung development Chen L.et al,Respiratory research,17(1): 1 Targeted inhibition of Focal Adhesion Kinase Attenuates Cardiac Fibrosis and Preserves Heart Function in Adverse Cardiac Remodeling Zhang J.et al,Sci Rep. ,Feb 22

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SKU	Supplier No.	Size	Your price
BHE10505422	CSB-EL005727MO	96T	$695	Add to Cart
BHE10505422	CSB-EL005727MO	5x96T	$3336	Add to Cart
BHE10505422	CSB-EL005727MO	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.