Mouse E-Cadherin,E-Cad ELISA Kit

Mfr.No.

CSB-E04520m

Sensitivity

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Detection Range

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Reactivity

Mouse

Assay Duration

1-5h

Sample Volume

50-100ul

Alternative Names

cadherin 1, type 1, E-cadherin (epithelial), Arc-1, CD324, CDHE, ECAD, LCAM, UVO, E-Cadherin, cadherin 1, E-cadherin (epithelial), cadherin 1, type 1, calcium-dependent adhesion protein, epithelial, cell-CAM 120/80, uvomorulin

Recovery

The recovery of rat RNLS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	82-91
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat RNLS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	106
	Range %	101-113
1:4	Average %	94
	Range %	90-98
1:8	Average %	89
	Range %	84-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
4000	2.204	2.132	2.168	1.972
2000	1.619	1.524	1.572	1.376
1000	1.002	0.948	0.975	0.779
500	0.628	0.663	0.646	0.450
250	0.430	0.407	0.419	0.223
125	0.295	0.281	0.288	0.092
62.5	0.241	0.250	0.246	0.050
0	0.193	0.199	0.196	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

UniProt ID	P09803
Tissue Specificity	Non-neural epithelial tissues.

Applications	ELISA
Research Area	CDH1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505617	CSB-E04520m	96T	$468	Add to Cart
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BHE10505617	CSB-E04520m	10x96T	$4446	Add to Cart

Mouse E-Cadherin,E-Cad ELISA Kit

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