Mouse ERK1/2(Extracellular Signal Regulated Kinase 1/2) ELISA Kit

Mfr.No.

ELK0019

Sensitivity

0.12 ng/mL

Detection Range

0.78-50 ng/mL

Assay Duration

3.5h

Assay Type

Sandwich

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ERK1/2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	85-94%	87-101%	86-97%	88-95%
EDTA Plasma(n=5)	85-94%	97-106%	92-101%	82-90%
Heparin Plasma(n=5)	95-103%	89-101%	87-98%	85-92%

Standard Curve

Concentration (ng/mL)	OD	Corrected OD
50.00	2.532	2.459
25.00	1.412	1.339
12.50	0.947	0.874
6.25	0.599	0.526
3.13	0.535	0.462
1.57	0.168	0.095
0.79	0.232	0.159
0.00	0.073	0.000

Sample Type

Tissue homogenates, Cell lysates, Other biological fluids

Limit Of Detection

50 ng/mL

Precision

Matrices listed below were spiked with certain level of recombinant ERK1/2 and the recovery rates were calculated by comparing the measured value to the expected amount of ERK1/2 in samples.

Matrix	Recovery range	Average
serum(n=5)	79-91%	85%
EDTA Plasma(n=5)	81-97%	89%
Heparin Plasma(n=5)	84-99%	91%

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ERK1/2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ERK1/2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ERK1/2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ERK1/2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Manual
MSDS

Research Area	Signal transduction;Enzyme & Kinase;Apoptosis;Tumor immunity;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15208904	ELK0019	48T	$320		Add to Cart
BHE15208904	ELK0019	96T	$458		Add to Cart

Mouse ERK1/2(Extracellular Signal Regulated Kinase 1/2) ELISA Kit

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