Mouse luteinizing hormone (LH) ELISA kit

The recovery of human MBP antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Serum (n=5)

Mfr.No.

CSB-E12770m

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Recovery

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MBP antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	85-103
1:2	Average %	95
	Range %	84-100
1:4	Average %	97
	Range %	90-105
1:8	Average %	92
	Range %	82-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Sample Type

Serum, Tissue Homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	O09108

Applications	ELISA
Research Area	LH

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Development and Characterization of Cell-Specific Androgen Receptor Knockout Mice /,The Nuclear Receptor Superfamily: Methods and Protocols,219-248. Exposure to Concentrated Ambient PM2. 5 Compromises Spermatogenesis in a Mouse Model: Role of Suppression of Hypothalamus-Pituitary-Gonads Axis Qiu L.et al,Toxicol Sci,Nov 20 Genotoxic and Hypogonadism Effect of Triclosan Treatment and the Mitigating Effect of Vitamin E in Male Albino Mice Nahed A Hussien.et al,Journal of Basic and Clinical Pharmacy,/ Reintroducing testosterone in the db/db mouse partially restores normal glucose metabolism and insulin resistance in a leptin-independent manner Koichi Yabiku.et al,BMC Endocrine Disorders,June 13

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