Mouse lymphotactin,Lptn/LTN ELISA Kit

Mouse lymphotactin,Lptn/LTN ELISA Kit

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Mfr.No.

CSB-E08714m

Sensitivity

0.039 ng/ml

Detection Range

0.156 ng/ml - 10 ng/ml

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

chemokine (C motif) ligand 1, ATAC, LPTN, LTN, SCM-1, SCM-1a, SCM1, SCYC1, lymphotactin, small inducible cytokine subfamily C, member 1 (lymphotactin)

Recovery

The recovery of rabbit F1+2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	83-92
EDTA plasma (n=4)	92	85-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit F1+2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-88
1:2	Average %	98
	Range %	92-104
1:4	Average %	97
	Range %	93-101
1:8	Average %	87
	Range %	82-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pmol/ml	OD1	OD2	Average	Corrected
20	2.989	2.788	2.889	2.786
10	2.535	2.372	2.454	2.351
5	1.781	1.815	1.798	1.695
2.5	1.002	1.012	1.007	0.904
1.25	0.485	0.506	0.496	0.393
0.625	0.301	0.311	0.306	0.203
0.312	0.178	0.175	0.177	0.074
0	0.103	0.102	0.103

Species

Mus musculus

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P47993
Tissue Specificity	Expressed in activated CD8(+) T cells. In the thymus, expressed by medullary thymic epithelial cells.

Applications	ELISA
Research Area	XCL1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506177	CSB-E08714m	96T	$468	Add to Cart
BHE10506177	CSB-E08714m	5x96T	$2246.40	Add to Cart
BHE10506177	CSB-E08714m	10x96T	$4446	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.