Mouse Lysozyme,LZM ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E11329m

Sensitivity

0.195 ng/mL

Detection Range

0.78 ng/mL-50 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

Lysozyme,LZM

Recovery

The recovery of rabbit SDF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	100-108
EDTA plasma (n=4)	93	91-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit SDF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	102
	Range %	88-106
1:2	Average %	95
	Range %	89-102
1:4	Average %	88
	Range %	85-94
1:8	Average %	99
	Range %	93-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	LZM

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Enhanced antimicrobial activity of engineered human lysozyme /,ACS Chem. Biol,2010 Sep 17;5(9):809-18. Standardized extract of Tinospora crispa stimulates innate and adaptive immune responses in Balb/c mice. Ahmad W. et al,Food Funct.,2016 Feb 3. Inhibitory Effects of the Standardized Extract of Phyllanthus amarus on Cellular and Humoral Immune Responses in Balb/C Mice. Ilangkovan M.et al,Phytother Res.,/

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