Mouse MAP1LC3a(Microtubule Associated Protein 1 Light Chain 3 Alpha) ELISA Kit

Mfr.No.

ELK9909

Sensitivity

0.19 ng/mL

Detection Range

0.78-50 ng/mL

Assay Duration

3.5h

Assay Type

Sandwich

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MAP1LC3a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	87-96%	91-101%	87-98%	89-103%
EDTA Plasma(n=5)	89-99%	83-96%	98-104%	88-101%
Heparin Plasma(n=5)	92-101%	82-98%	89-97%	85-93%

Standard Curve

Concentration (ng/mL)	OD	Corrected OD
50.00	2.119	2.023
25.00	1.527	1.431
12.50	1.236	1.140
6.25	0.815	0.719
3.13	0.593	0.497
1.57	0.329	0.233
0.79	0.197	0.101
0.00	0.096	0.000

Sample Type

Tissue homogenates, cell lysates and other biological fluids

Limit Of Detection

50 ng/mL

Precision

Matrices listed below were spiked with certain level of recombinant MAP1LC3a and the recovery rates were calculated by comparing the measured value to the expected amount of MAP1LC3a in samples.

Matrix	Recovery range	Average
serum(n=5)	87-99%	93%
EDTA Plasma(n=5)	78-92%	85%
Heparin Plasma(n=5)	92-107%	99%

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MAP1LC3a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MAP1LC3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MAP1LC3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MAP1LC3a in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Manual
MSDS

UniProt ID	Q91VR7

Research Area	Metabolic pathway;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15210387	ELK9909	48T	$320		Add to Cart
BHE15210387	ELK9909	96T	$458		Add to Cart

Mouse MAP1LC3a(Microtubule Associated Protein 1 Light Chain 3 Alpha) ELISA Kit

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