Mouse Oncostatin-M,OSM ELISA KIT

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E04697m

Alternative Names

oncostatin M, MGC20461

Reactivity

Mouse

Detection Range

3.12 pg/mL-200 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse ON spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-104
EDTA plasma (n=4)	91	87-95

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ON in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	86
	Range %	83-89
1:200	Average %	87
	Range %	82-95
1:400	Average %	96
	Range %	94-98
1:800	Average %	97
	Range %	91-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Sensitivity

0.78 pg/mL

Limit Of Detection

450 nm

UniProt ID	P53347
Antigen	Oncostatin M

Applications

ELISA

Research Area

OSM

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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