Mouse Osteoprotegerin,OPG ELISA KIT

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E04693m

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

tumor necrosis factor receptor superfamily, member 11b, MGC29565, OCIF, OPG, TR1, osteoclastogenesis inhibitory factor, osteoprotegerin

Recovery

The recovery of human Pro-ADM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-100
EDTA plasma (n=4)	101	90-110

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human Pro-ADM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:200	Average %	85
	Range %	80-92
1:400	Average %	95
	Range %	90-100
1:800	Average %	99
	Range %	92-110
1:1600	Average %	92
	Range %	82-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	O08712
Tissue Specificity	Highly expressed in liver, lung, stomach, intestines and calvaria. Highly expressed in decidua and placenta, and in embryo.

Applications	ELISA
Research Area	TNFRSF11B

Storage	Store at 2-8°C. Please refer to protocol.

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