Mouse PIEZO-1(Piezo-Type mMechanosensitive iIon Channel Component 1) ELISA Kit

Mfr.No.

ELK0282

Sensitivity

22.3 pg/mL

Detection Range

78.13-5000 pg/mL

Assay Duration

3.5h

Assay Type

Sandwich

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PIEZO-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	89-102%	82-94%	85-96%	78-90%
EDTA Plasma(n=5)	85-94%	97-106%	82-95%	93-105%
Heparin Plasma(n=5)	85-92%	83-96%	78-98%	90-99%

Standard Curve

Concentration (pg/mL)	OD	Corrected OD
5000.00	2.368	2.287
2500.00	1.585	1.504
1250.00	1.115	1.034
625.00	0.892	0.811
312.50	0.563	0.482
156.25	0.368	0.287
78.13	0.218	0.137
0.00	0.081	0.000

Sample Type

Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates, Other biological fluids

Limit Of Detection

5000 pg/mL

Precision

Matrices listed below were spiked with certain level of recombinant PIEZO-1 and the recovery rates were calculated by comparing the measured value to the expected amount of PIEZO-1 in samples.

Matrix	Recovery range	Average
serum(n=5)	86-99%	92%
EDTA Plasma(n=5)	89-103%	96%
Heparin Plasma(n=5)	83-97%	90%

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PIEZO-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PIEZO-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PIEZO-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PIEZO-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Manual
MSDS

UniProt ID	E2JF22

Research Area	Signal transduction;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15209139	ELK0282	48T	$320		Add to Cart
BHE15209139	ELK0282	96T	$458		Add to Cart

Mouse PIEZO-1(Piezo-Type mMechanosensitive iIon Channel Component 1) ELISA Kit

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