Mouse Progesterone,PROG ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E05104m

Sensitivity

0.2 ng/mL

Detection Range

0.3 ng/mL-10 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Competitive

Sample Volume

50-100ul

Alternative Names

Progesterone,PROG

Recovery

The recovery of human ADH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	85-98
EDTA plasma (n=4)	94	90-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ADH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	83
	Range %	81-99
1:2	Average %	95
	Range %	90-105
1:4	Average %	85
	Range %	80-91
1:8	Average %	92
	Range %	80-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Sample Type

Serum, Plasma

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	PROG

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Anthrax Toxin Receptor 2 Functions in ECM Homeostasis of the Murine Reproductive Tract and Promotes MMP Activity Reeves CV et al ,PLOS ONE,2012 7(4):e34862 Superovulation Induced Changes of Lipid Metabolism in Ovaries and Embryos and Its Probable Mechanism Wang LY. et al,PLoS One,2015 Jul 13;10(7):e0132638. Bisphenol A Initiates Excessive Premature Activation of Primordial Follicles in Mouse Ovaries via the PTEN Signaling Pathway Hu Y.et al,Reprod Sci.,Oct 5 Silica nanoparticle exposure inducing granulosa cell apoptosis and follicular atresia in female Balb/c mice Liu J.et al,Environ Sci Pollut Res Int,Nov 19

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