Mouse PYCARD(PYD And CARD Domain Containing Protein) ELISA Kit

Mfr.No.

ELK8445

Alternative Names

ASC; CARD5; TMS1; Apoptosis-associated speck-like protein containing a CARD; Caspase recruitment domain-containing protein 5; Target of methylation-induced silencing 1

Reactivity

Mouse

Detection Range

7.82-500 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Other biological fluids, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant PYCARD and the recovery rates were calculated by comparing the measured value to the expected amount of PYCARD in samples.

Matrix	Recovery range	Average
serum(n=5)	82-97%	89%
EDTA plasma(n=5)	90-105%	97%
Heparin plasma(n=5)	88-102%	95%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PYCARD and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	79-96%	85-99%	79-93%	89-97%
EDTA plasma(n=5)	87-96%	86-98%	91-103%	87-90%
Heparin plasma(n=5)	87-98%	85-96%	92-102%	81-93%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
500.00	2.126	2.018
250.00	1.529	1.421
125.00	1.175	1.067
62.50	0.891	0.783
31.25	0.567	0.459
15.63	0.324	0.216
7.82	0.256	0.148
0.00	0.108	0.000

Sensitivity

3 pg/mL

Limit Of Detection

500 pg/mL

UniProt ID	Q9EPB4
Antigen	PYCARD
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PYCARD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PYCARD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PYCARD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PYCARD in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Apoptosis;
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE15205789	ELK8445	48T	$320		Add to Cart
BHE15205789	ELK8445	96T	$458		Add to Cart

Mouse PYCARD(PYD And CARD Domain Containing Protein) ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Mouse PYCARD(PYD And CARD Domain Containing Protein) ELISA Kit

Rate it now..!!