Mouse Semaphorin-3D - SEMA3D ELISA Kit

Mfr.No.

E2449Mo

Alternative Names

SEMA 3D;SEMA3D;Semaphorin-3D

Reactivity

Mouse

Detection Range

15-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

89549

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

7.38ng/L

Background

Induces the collapse and paralysis of neuronal growth cones. Could potentially act as repulsive cues toward specific neuronal populations. Binds to neuropilin (By similarity).

Gene ID	108151
UniProt ID	Q8BH34
Antigen	SEMA3D

Research Area

Neuroscience

Protocol

These standard curves of E2449Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.071	2.061	2.008
	2.05
800ng/L	1.913	1.893	1.841
	1.874
400ng/L	1.173	1.175	1.122
	1.176
200ng/L	0.728	0.668	0.615
	0.608
100ng/L	0.41	0.399	0.346
	0.388
0ng/L	0.061	0.053	0
	0.044

Precision

We measured random samples of E2449Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	332	20.35	6.1
Intra-Assay	2	18	402.7	13.16	3.3
Intra-Assay	3	18	131.5	5.9	4.5

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110260	E2449Mo	48T	$320		Add to Cart
BHE12110260	E2449Mo	96T	$458		Add to Cart

Mouse Semaphorin-3D - SEMA3D ELISA Kit

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