Mouse Stem cell factor/mast cell growth factor,SCF/MGF ELISA kit

Mouse Stem cell factor/mast cell growth factor,SCF/MGF ELISA kit

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Mfr.No.

CSB-E04719m

Sensitivity

0.78 pg/mL

Detection Range

3.12 pg/mL-200 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

KIT ligand, DKFZp686F2250, FPH2, KL-1, Kitl, MGF, SCF, SF, SHEP7, familial progressive hyperpigmentation 2, mast cell growth factor, steel factor, stem cell factor

Recovery

The recovery of rat RF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	83-95
EDTA plasma (n=4)	99	92-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat RF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	104
	Range %	96-108
1:10	Average %	94
	Range %	91-98
1:20	Average %	102
	Range %	98-106
1:40	Average %	88
	Range %	82-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average	Corrected
1000	2.646	2.545	2.596	2.443
500	1.975	2.076	2.026	1.873
250	1.284	1.308	1.296	1.143
125	0.751	0.762	0.757	0.604
62.5	0.512	0.501	0.507	0.354
31.2	0.353	0.342	0.348	0.195
15.6	0.285	0.281	0.283	0.130
0	0.153	0.152	0.153	?

Species

Mus musculus

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P20826
Tissue Specificity	Expressed in the cochlea.

Applications	ELISA
Research Area	KITLG

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506726	CSB-E04719m	96T	$615	Add to Cart
BHE10506726	CSB-E04719m	5x96T	$2952	Add to Cart
BHE10506726	CSB-E04719m	10x96T	$5842.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.