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Home / ELISA Kit

Mouse TGF-beta receptor type-1 (TGFBR1) ELISA Kit

SKU : BHE13705531

Tgfbr1 encodes a member of the transforming growth factor beta (TGF-beta) receptor family of proteins. These proteins comprise one component of the TGF-beta signaling pathway, which transduces extracellular signals into gene expression changes to regulate a wide range of cellular responses, including proliferation, migration, differentiation and apoptosis. Homozygous knockout mice for Tgfbr1 exhibit impaired angiogenesis and embryonic lethality. Alternative splicing results in multiple transcript variants.

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Mfr.No.	KTE70372
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse TGF-beta receptor type-1 (TGFBR1) ELISA Kit employs a two-site sandwich ELISA to quantitate TGFBR1.
Alternative Names	TGFBR1; AAT5; ACVRLK4; ALK-5; ALK5; LDS1A; LDS2A; SKR4; TGFR-1; activin A receptor type-like kinase; 53kD; activin A receptor type-like kinase; 53kDa; activin receptor-like kinase 5; serine/threonine-pro
Detection Method	Colorimetric
Kit Components	Mouse TGF-beta receptor type-1 microplate Mouse TGF-beta receptor type-1 standard Mouse TGF-beta receptor type-1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse TGF-beta receptor type-1 (TGFBR1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TGFBR1. No significant cross-reactivity or interference between Mouse TGFBR1 and analogues was observed.
Calibration Range	Please inquire
Sample Type	Serum, Cell culture supernatants, Plasma, Other biological fluids
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Tgfbr1 encodes a member of the transforming growth factor beta (TGF-beta) receptor family of proteins. These proteins comprise one component of the TGF-beta signaling pathway, which transduces extracellular signals into gene expression changes to regulate a wide range of cellular responses, including proliferation, migration, differentiation and apoptosis. Homozygous knockout mice for Tgfbr1 exhibit impaired angiogenesis and embryonic lethality. Alternative splicing results in multiple transcript variants.
Notes	This Mouse TGF-beta receptor type-1 (TGFBR1) ELISA Kit employs a two-site sandwich ELISA to quantitate TGFBR1 in samples. An antibody specific for TGFBR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTGFBR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGFBR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGFBR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	21812

Applications	ELISA
Applications Notes	This Mouse TGF-beta receptor type-1 (TGFBR1) ELISA Kit employs a two-site sandwich ELISA to quantitate TGFBR1 in samples. An antibody specific for TGFBR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTGFBR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGFBR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGFBR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705531	KTE70372	48 T	$339		Add to Cart
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BHE13705531	KTE70372	96 Tx50	$24569		Add to Cart

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