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Home / ELISA Kit

Mouse Toll/Interleukin-1 receptor domain-containing adapter protein (TIRAP) ELISA Kit

SKU : BHE13705381

TIRAP is a TIR adaptor protein involved in the TLR4 signaling pathway of the immune system. It activates NF-kappa-B, MAPK1, MAPK3 and JNK, which then results in cytokine secretion and the inflammatory response. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described. MAL, unlike MYD88, does not interact with IRAK1 and is not inhibited by the dominant-negative N-terminal region of IRAK1; however, like MYD88, MAL does, through its TIR domain, interact with and is inhibited in NFKB activation by the dominant-negative form of IRAK2. A dominant-negative form of MAL inhibits TLR4 or lipopolysaccharide activation of NFKB, but not NFKB activation by IL1R1 or IL18R. Immunoprecipitation analysis showed that TLR4 and MAL are constitutively associated.

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Mfr.No.	KTE70221
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Toll/Interleukin-1 receptor domain-containing adapter protein (TIRAP) ELISA Kit employs a two-site sandwich ELISA to quantitate TIRAP.
Alternative Names	TIRAP; FLJ42305; Mal; wyatt; MyD88 adapter-like protein; OTTHUMP00000179130; Toll-interleukin 1 receptor domain-containing adaptor protein; Toll-like receptor adaptor protein; adapter protein wyatt
Detection Method	Colorimetric
Kit Components	Mouse Toll/Interleukin-1 receptor domain-containing adapter protein microplate Mouse Toll/Interleukin-1 receptor domain-containing adapter protein standard Mouse Toll/Interleukin-1 receptor domain-containing adapter protein detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Toll/Interleukin-1 receptor domain-containing adapter protein (TIRAP) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TIRAP. No significant cross-reactivity or interference between Mouse TIRAP and analogues was observed.
Calibration Range	Please inquire
Sample Type	Serum, Cell culture supernatants, Plasma, Other biological fluids
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TIRAP is a TIR adaptor protein involved in the TLR4 signaling pathway of the immune system. It activates NF-kappa-B, MAPK1, MAPK3 and JNK, which then results in cytokine secretion and the inflammatory response. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described. MAL, unlike MYD88, does not interact with IRAK1 and is not inhibited by the dominant-negative N-terminal region of IRAK1; however, like MYD88, MAL does, through its TIR domain, interact with and is inhibited in NFKB activation by the dominant-negative form of IRAK2. A dominant-negative form of MAL inhibits TLR4 or lipopolysaccharide activation of NFKB, but not NFKB activation by IL1R1 or IL18R. Immunoprecipitation analysis showed that TLR4 and MAL are constitutively associated.
Notes	This Mouse Toll/Interleukin-1 receptor domain-containing adapter protein (TIRAP) ELISA Kit employs a two-site sandwich ELISA to quantitate TIRAP in samples. An antibody specific for TIRAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTIRAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIRAP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIRAP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	117149

Applications	ELISA
Applications Notes	This Mouse Toll/Interleukin-1 receptor domain-containing adapter protein (TIRAP) ELISA Kit employs a two-site sandwich ELISA to quantitate TIRAP in samples. An antibody specific for TIRAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTIRAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIRAP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIRAP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705381	KTE70221	48 T	$339		Add to Cart
BHE13705381	KTE70221	96 T	$589		Add to Cart
BHE13705381	KTE70221	96 Tx5	$2629		Add to Cart
BHE13705381	KTE70221	96 Tx50	$24569		Add to Cart

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