Mouse Tryptase beta-2(TPSB2) ELISA kit

Mouse Tryptase beta-2(TPSB2) ELISA kit

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Mfr.No.

CSB-EL024128MO

Sensitivity

19.5 pg/mL

Detection Range

78 pg/mL-5000 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

tryptase beta 2, TPS2, tryptaseB, tryptaseC, OTTHUMP00000208944, mast cell tryptase beta II, mast cell tryptase beta III, tryptase II, tryptase III, tryptase beta 2, tryptase beta II, tryptase beta III

Recovery

The recovery of human HPC? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-101
EDTA plasma (n=4)	97	92-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human HPC? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-92
1:2	Average %	97
	Range %	91-105
1:4	Average %	93
	Range %	90-96
1:8	Average %	95
	Range %	89-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
300	1.356	1.342	1.349
200	0.967	1.023	0.995
100	0.466	0.482	0.474
50	0.211	0.199	0.205
25	0.050	0.048	0.049

Species

Mus musculus

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P21845
Tissue Specificity	During embryogenesis, detected primarily in skin.

Applications	ELISA
Research Area	TPSB2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506849	CSB-EL024128MO	96T	$695	Add to Cart
BHE10506849	CSB-EL024128MO	5x96T	$3336	Add to Cart
BHE10506849	CSB-EL024128MO	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<20%

Three samples of known concentration were tested in twenty assays to assess.