Pig free thyroxine (FT4) ELISA kit

Mfr.No.

CSB-EQF027512PI

Sensitivity

1.08 pmol/L

Detection Range

4 pmol/L-64 pmol/L

Reactivity

Pig

Assay Duration

1-5h

Assay Type

Competitive

Sample Volume

50-100ul

Alternative Names

free thyroxine (FT4)

Recovery

The recovery of human FHIT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-100
EDTA plasma (n=4)	101	96-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human FHIT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	87
	Range %	82-91
1:4	Average %	95
	Range %	90-100
1:8	Average %	84
	Range %	80-88

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Sus scrofa

Sample Type

Serum, Cell culture supernatants, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	FT4

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10507077	CSB-EQF027512PI	10x96T	$4702.50	Add to Cart