Pig Proinsulin,PI ELISA Kit

Mfr.No.

CSB-E13262p

Alternative Names

Proinsulin,PI

Reactivity

Pig

Detection Range

62.5 pg/mL-4000 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human SPP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	105	99-108
EDTA plasma (n=4)	94	90-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SPP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	98
	Range %	96-102
1:10	Average %	107
	Range %	103-114
1:20	Average %	94
	Range %	90-98
1:40	Average %	88
	Range %	86-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Sus scrofa

Sensitivity

15.6 pg/mL

Limit Of Detection

450 nm

Antigen	Proinsulin,PI

Applications

ELISA

Research Area

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507214	CSB-E13262p	96T	$595	Add to Cart
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BHE10507214	CSB-E13262p	10x96T	$5652.50	Add to Cart