Pig reproductive and respiratory syndrome virus(PRRSV) antibody(IgG)ELISA kit

Mfr.No.

CSB-EQ027198PI

Reactivity

Pig

Assay Duration

1-5h

Sample Volume

50-100ul

Alternative Names

reproductive and respiratory syndrome virus(PRRSV)antibody(IgG)

Recovery

The recovery of human C8B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	85-104
EDTA plasma (n=4)	91	88-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human C8B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:10000	Average %	92
	Range %	89-98
1:20000	Average %	90
	Range %	84-100
1:40000	Average %	95
	Range %	92-101
1:80000	Average %	93
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Sus scrofa

Sample Type

Serum

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	IgG

Storage	Store at 2-8°C. Please refer to protocol.

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