Pig Tumor necrosis factor (TNF/TNFA/TNFSF2) ELISA kit

Mfr.No.

CSB-E16980p

Sensitivity

0.040 ng/mL

Detection Range

0.047 ng/mL-3 ng/mL

Reactivity

Pig

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

Tumor necrosis factor (TNF/TNFA/TNFSF2), DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2, APC1 protein, TNF superfamily, member 2, TNF, macrophage-derived, TNF, monocyte-derived, cachectin, tumor necrosis factor alpha

Recovery

The recovery of rat PPY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	83-94
EDTA plasma (n=4)	100	91-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat PPY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	104
	Range %	93-109
1:200	Average %	100
	Range %	91-104
1:400	Average %	97
	Range %	88-101
1:800	Average %	90
	Range %	82-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
50	2.069	1.932	2.001	1.899
25	1.429	1.466	1.448	1.346
12.5	0.946	0.917	0.932	0.830
6.25	0.662	0.651	0.657	0.555
3.12	0.348	0.340	0.344	0.242
1.56	0.242	0.237	0.240	0.138
0.78	0.164	0.159	0.162	0.060
0	0.104	0.100	0.102	?

Species

Sus scrofa

Sample Type

Serum, Cell culture supernatants, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P23563

Applications	ELISA
Research Area	TNF

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507265	CSB-E16980p	96T	$495	Add to Cart
BHE10507265	CSB-E16980p	5x96T	$2376	Add to Cart
BHE10507265	CSB-E16980p	10x96T	$4702.50	Add to Cart

Pig Tumor necrosis factor (TNF/TNFA/TNFSF2) ELISA kit

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