Porcine Endothelial Nitric Oxide Synthase

Mfr.No.

E0231Po

Sensitivity

0.55IU/mL

Detection Range

1-400IU/mL

Reactivity

Porcine

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

cNOS;Constitutive NOS;EC-NOS;Endothelial NOS;eNOS;Nitric oxide Synthase, endothelial;NOS 3;NOS type III;NOS3;NOSIII

Molecular Weight

133406

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0231Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	35.2	2.52	7.1
Intra-Assay	2	18	88.9	5.34	6
Intra-Assay	3	18	161.4	5.6	3.5

Background

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets (By similarity).

Protocol

These standard curves of E0231Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
240IU/mL	2.289	2.177	2.13
	2.064
120IU/mL	1.183	1.221	1.175
	1.26
60IU/mL	0.856	0.77	0.723
	0.683
30IU/mL	0.483	0.472	0.426
	0.462
15IU/mL	0.241	0.247	0.201
	0.253
0IU/mL	0.04	0.046	0
	0.052

Gene ID	397557
UniProt ID	Q28969
Antigen	NOS3

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110639	E0231Po	48T	$320		Add to Cart
BHE12110639	E0231Po	96T	$458		Add to Cart

Porcine Endothelial Nitric Oxide Synthase - ENOS ELISA Kit

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