Porcine Thyrotropin-releasing Hormone

Mfr.No.

E0182Po

Sensitivity

2.43ng/L

Detection Range

5-1000ng/L

Reactivity

Porcine

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of thyrotropin-releasing hormone (also known as TRH) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Protirelin;Thyroliberin;Thyrotropin-releasing factor;Thyrotropin-releasing hormone;TRF;TRH;TSH-releasing factor

Molecular Weight

380

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E0182Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	518.61	22.23	4.29
Intra-Assay	2	18	316.19	13.19	4.17
Intra-Assay	3	18	122.38	6.05	4.94

Background

Functions as a regulator of the biosynthesis of TSH in the anterior pituitary gland and as a neurotransmitter/ neuromodulator in the central and peripheral nervous systems.

Protocol

These standard curves of E0182Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
640ng/L	1.688	1.738	1.684
	1.787
320ng/L	1.137	1.078	1.025
	1.019
160ng/L	0.435	0.512	0.459
	0.59
80ng/L	0.265	0.243	0.189
	0.22
40ng/L	0.121	0.122	0.069
	0.123
0ng/L	0.051	0.053	0
	0.056

Gene ID	100513309
UniProt ID	P62968

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110599	E0182Po	48T	$320		Add to Cart
BHE12110599	E0182Po	96T	$458		Add to Cart

Porcine Thyrotropin-releasing Hormone - TRH ELISA Kit

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