Rabbit Platelet-Derived Growth Factor AB(PDGF-AB)ELISA Kit

Mfr.No.

CSB-E15910Rb

Alternative Names

Platelet-Derived Growth Factor AB(PDGF-AB)

Reactivity

Rabbit

Detection Range

1.56 ng/mL-100 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human SPINK6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-106
EDTA plasma (n=4)	104	88-110

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SPINK6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-93
1:2	Average %	98
	Range %	94-102
1:4	Average %	102
	Range %	98-106
1:8	Average %	95
	Range %	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	1.712	1.812	1.762	1.626
20	1.352	1.366	1.359	1.223
10	0.966	0.954	0.960	0.824
5	0.587	0.598	0.593	0.457
2.5	0.463	0.448	0.456	0.320
1.25	0.323	0.345	0.334	0.198
0.625	0.217	0.221	0.219	0.083
0	0.135	0.136	0.136	?

Species

Oryctolagus cuniculus

Sensitivity

0.39 ng/mL

Limit Of Detection

450 nm

Antigen	Platelet-Derived Growth Factor AB(PDGF-AB)

Applications

ELISA

Research Area

PDGF-AB

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Surface functionalized electrospun fibrous poly (3-hydroxybutyrate) membranes and sleeves: a novel approach for fixation in anterior cruciate ligament reconstruction Wei-Chuan Chen.et al,J. Mater. Chem. B, ,Dec 9 Autologous platelet-rich plasma induces bone formation of tissue-engineered bone with boneMarrow mesenchymal stem cells on beta-tricalcium phosphate Yu T.et al,J Orthop Surg Res,Nov 21

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SKU	Supplier No.	Size	Your price
BHE10507436	CSB-E15910Rb	96T	$595	Add to Cart
BHE10507436	CSB-E15910Rb	5x96T	$2856	Add to Cart
BHE10507436	CSB-E15910Rb	10x96T	$5652.50	Add to Cart

Rabbit Platelet-Derived Growth Factor AB(PDGF-AB)ELISA Kit

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