Matrices listed below were spiked with certain level of Bax and the recovery rates were calculated by comparing the measured value to the expected amount of Bax in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-103
92
EDTA plasma(n=5)
88-105
95
heparin plasma(n=5)
88-95
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Bax and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Bax/apoptosis regulator BAX/BCL2-associated X protein/Bcl2-L-4/BCL2L4bcl2-L-4/Bcl-2-like protein 4
Notes
For Research Use Only
UniProt ID
Q63690
Antigen
Bax
Applications
ELISA
Applications Notes
Bax ELISA Kit allows for the in vitro quantitative determination of Bax concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat Bax(Apoptosis regulator BAX) ELISA Kit