Rat C1qB(Complement Component 1, Q Subcomponent B) ELISA Kit

Mfr.No.

ELK7531

Sensitivity

0.64 ng/mL

Detection Range

1.57-100 ng/mL

Reactivity

Rat

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

Complement Component 1,q Subcomponent,Beta Polypeptide

Recovery

Matrices listed below were spiked with certain level of recombinant C1qB and the recovery rates were calculated by comparing the measured value to the expected amount of C1qB in samples.

Matrix	Recovery range	Average
serum(n=5)	81-95%	88%
EDTA plasma(n=5)	87-99%	93%
Heparin plasma(n=5)	87-99%	93%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C1qB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	86-93%	91-105%	87-98%	89-94%
EDTA plasma(n=5)	79-96%	85-99%	79-93%	89-97%
Heparin plasma(n=5)	85-94%	87-101%	89-99%	85-95%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
100.00	2.038	1.937
50.00	1.593	1.492
25.00	1.243	1.142
12.50	0.796	0.695
6.25	0.583	0.482
3.13	0.297	0.196
1.57	0.226	0.125
0.00	0.101	0.000

Sample Type

Serum, Plasma, Other biological fluids

Limit Of Detection

100 ng/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat C1qB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat C1qB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat C1qB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat C1qB in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

UniProt ID	P31721

Applications	ELISA
Research Area	Immune molecule;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15204602	ELK7531	48T	$320		Add to Cart
BHE15204602	ELK7531	96T	$458		Add to Cart

Rat C1qB(Complement Component 1, Q Subcomponent B) ELISA Kit

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