Rat Calpain 3 - CAPN3 ELISA Kit

Mfr.No.

E1803Ra

Sensitivity

0.045ng/ml

Detection Range

0.1-40ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat CALPAIN 3 (also known as CAPN3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Calcium-activated neutral proteinase 3;Calpain L3;Calpain p94;Calpain-3;CANP 3;CAPN3;Lp82;Lp84;Lp85;Muscle-specific calcium-activated neutral protease 3;p94

Molecular Weight

94127

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E1803Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	16.82	0.49	2.92
Intra-Assay	2	18	8.16	0.29	3.6
Intra-Assay	3	18	11.77	0.59	4.99

Background

Calcium-regulated non-lysosomal thiol-protease. Proteolytically cleaves CTBP1 at 'His-399'. Mediates, with UTP25, the proteasome-independent degradation of p53/TP53.

Protocol

These standard curves of E1803Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	1.941	1.984	1.942
	2.027
12ng/ml	1.041	1.056	1.015
	1.071
6ng/ml	0.518	0.495	0.454
	0.471
3ng/ml	0.234	0.254	0.213
	0.275
1.5ng/ml	0.125	0.133	0.092
	0.14
0ng/ml	0.035	0.041	0
	0.048

Gene ID	29155
UniProt ID	P16259

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113004	E1803Ra	48T	$320		Add to Cart
BHE12113004	E1803Ra	96T	$458		Add to Cart

Rat Calpain 3 - CAPN3 ELISA Kit

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