Rat Caspase 3,Casp-3 ELISA Kit

Rat Caspase 3,Casp-3 ELISA Kit

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Mfr.No.

CSB-E08857r

Sensitivity

0.078 ng/mL

Detection Range

0.312 ng/mL-20 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

caspase 3, apoptosis-related cysteine peptidase, CPP32, CPP32B, SCA-1, PARP cleavage protease, SREBP cleavage activity 1, Yama, apopain, caspase 3, caspase 3, apoptosis-related cysteine protease, cysteine protease CPP32, procaspase3

Recovery

The recovery of mouse PTGDS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	95-109
EDTA plasma (n=4)	92	87-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PTGDS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-97
1:2	Average %	84
	Range %	80-88
1:4	Average %	95
	Range %	90-100
1:8	Average %	98
	Range %	94-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
300	2.458	2.352	2.405	2.238
150	1.715	1.806	1.761	1.594
75	1.098	1.054	1.076	0.909
37.5	0.604	0.643	0.624	0.457
18.8	0.356	0.371	0.364	0.197
9.4	0.267	0.260	0.264	0.097
4.7	0.221	0.235	0.228	0.061
0	0.165	0.169	0.167

Species

Rattus norvegicus

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P55213
Tissue Specificity	Expressed in heart, brain, liver, and muscle but not in kidney or testis.

Applications	ELISA
Research Area	CASP3

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507728	CSB-E08857r	96T	$615	Add to Cart
BHE10507728	CSB-E08857r	5x96T	$2952	Add to Cart
BHE10507728	CSB-E08857r	10x96T	$5842.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.