Rat Collagen alpha-1(I) chain (COL1A1) ELISA kit

Rat Collagen alpha-1(I) chain (COL1A1) ELISA kit

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Mfr.No.

CSB-E12732r

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

collagen, type I, alpha 1, OI4, alpha 1 type I collagen, collagen alpha 1 chain type I, collagen of skin, tendon and bone, alpha-1 chain, pro-alpha-1 collagen type 1,_x000D_ Collagen alpha-1(1) chain (COL1A1),_x000D_ Collagen alpha-1(I) chain (COL1A1), collagen, type I, alpha 1, OI4, alpha 1 type I collagen, collagen alpha 1 chain type I, collagen of skin, tendon and bone, alpha-1 chain, pro-alpha-1 collagen type 1,_x000D_ Collagen alpha-1(1) chain (COL1A1),_x000D_ Collagen alpha-1(I) chain (COL1A1)

Recovery

The recovery of human KAF/AR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93
EDTA plasma (n=4)	104	100-108

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human KAF/AR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	86-97
1:2	Average %	83
	Range %	80-86
1:4	Average %	91
	Range %	85-97
1:8	Average %	98
	Range %	95-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
300	2.812	2.836	2.824	2.681
150	1.971	1.965	1.968	1.825
75	1.122	1.168	1.145	1.002
37.5	0.590	0.571	0.581	0.438
18.8	0.385	0.399	0.392	0.249
9.4	0.293	0.273	0.283	0.140
4.7	0.210	0.216	0.213	0.070
0	0.142	0.144	0.143

Species

Rattus norvegicus

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P02456
Tissue Specificity	Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.

Applications	ELISA
Research Area	COL1A1

Storage	Store at 2-8°C. Please refer to protocol.

Reference	iTRAQ-based proteomic analysis of combination therapy with taurine, epigallocatechin gallate, and genistein on carbon tetrachloride-induced liver fibrosis in rats. Cao W.et al,Toxicol Lett.,232(1):233-45 Lentiviral Vector-Mediated FoxO1 Overexpression Inhibits Extracellular Matrix Protein Secretion Under High Glucose Conditions in Mesangial Cells. Guo F.et al,J Cell Biochem.,2016 117(1):74-83.

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SKU	Supplier No.	Size	Your price
BHE10507788	CSB-E12732r	96T	$835	Add to Cart
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BHE10507788	CSB-E12732r	10x96T	$7932.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.